https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Luminal Mg²⁺, a key factor controlling RYR2-mediated Ca²⁺ release: cytoplasmic and luminal regulation modeled in a tetrameric channel https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:4426 Wed 11 Apr 2018 16:18:36 AEST ]]> A cell cycle-associated change in Ca²⁺ releasing activity leads to the generation of Ca²⁺ transients in mouse embryos during the first mitotic division https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6547 Wed 11 Apr 2018 15:10:36 AEST ]]> Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6545 Wed 11 Apr 2018 14:52:54 AEST ]]> Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10991 Wed 11 Apr 2018 14:47:28 AEST ]]> Luminal Ca²⁺-regulated Mg²⁺ inhibition of skeletal RyRs reconstituted as isolated channels or coupled clusters https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1802 Wed 11 Apr 2018 13:46:30 AEST ]]> IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:4425 Wed 11 Apr 2018 13:44:07 AEST ]]> Expansion of somatically reverted memory CD8(+) T cells in patients with X-linked lymphoproliferative disease caused by selective pressure from Epstein-Barr virus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23657 Wed 11 Apr 2018 13:34:30 AEST ]]> Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6956 Wed 11 Apr 2018 10:22:18 AEST ]]> Dantrolene inhibition of ryanodine channels (RyR2) in artificial lipid bilayers depends on FKBP12.6 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53989 Thu 25 Jan 2024 13:03:51 AEDT ]]> Differences in the regulation of RyR2 from human, sheep, and rat by Ca²⁺ and Mg²⁺ in the cytoplasm and in the lumen of the sarcoplasmic reticulum https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18980 i = 10 µM at 100 nM Ca²⁺) that was similar to RyR2 from rat and sheep obtained under the same experimental conditions. However, in the presence of 0.1 mM Ca²⁺, RyR2s from human were 3.5-fold less sensitive to cytoplasmic Mg²⁺ inhibition than those from sheep and rat. The Kₐ values for luminal Ca²⁺ activation were similar in the three species (35 µM for human, 12 µM for sheep, and 10 µM for rat). From the relationship between open probability and luminal [Ca²⁺], the peak open probability for the human RyR2 was approximately the same as that for sheep, and both were ~10-fold greater than that for rat RyR2. Human RyR2 also showed the same sensitivity to luminal Mg²⁺ as that from sheep, whereas rat RyR2 was 10-fold more sensitive. In all species, modulation of RyR2 gating by luminal Ca²⁺ and Mg²⁺ only occurred when cytoplasmic [Ca²⁺] was <3 µM. The activation response of RyR2 to luminal and cytoplasmic Ca²⁺ was strongly dependent on the Mg²⁺ concentration. Addition of physiological levels (1 mM) of Mg²⁺ raised the Kₐ for cytoplasmic Ca²⁺ to 30 µM (human and sheep) or 90 µM (rat) and raised the Kₐ for luminal Ca²⁺ to ~1 mM in all species. This is the first report of the regulation by Ca²⁺ and Mg²⁺ of native RyR2 receptor activity from healthy human hearts.]]> Sat 24 Mar 2018 07:58:52 AEDT ]]>